The Mouse FETUA (Fetuin A) ELISA Kit is meticulously designed for the precise and quantitative measurement of Fetuin A levels in a variety of biological samples. Fetuin A, a multifunctional glycoprotein also known as alpha-2-HS-glycoprotein, plays vital roles in regulating diverse physiological processes. Fetuin A is recognized for its involvement in various cellular functions, including inhibition of calcification, modulation of insulin signaling, and regulation of inflammatory responses. Accurate measurement of Fetuin A levels is fundamental in understanding its implications in metabolic disorders, bone health, and inflammatory conditions. Fetuin A serves as a biomarker with diagnostic and prognostic relevance in several diseases, making it an essential target for research in biomedical and clinical settings. The Mouse FETUA ELISA Kit from Assay Genie offers exceptional sensitivity and specificity, ensuring dependable and reproducible results. Manufactured under strict quality standards, this kit delivers robust performance and user-friendly operation, making it an excellent choice for researchers investigating the functions and significance of Fetuin A in biological systems.
Product Name:
Mouse FETUA (Fetuin A) ELISA Kit
SKU:
AEES00258
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 ng/mL
Detection range:
78.12-5000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
