The Mouse FN (Fibronectin) CLIA Kit is a powerful tool for researchers looking to accurately measure fibronectin levels in mouse serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring that you can rely on precise and reproducible results for your experiments.Fibronectin is a key component of the extracellular matrix and plays a critical role in cell adhesion, migration, and wound healing processes. Dysregulation of fibronectin levels has been linked to various diseases, including cancer, cardiovascular disorders, and fibrotic conditions.
By using the Mouse FN CLIA Kit, researchers can gain valuable insights into the role of fibronectin in disease pathogenesis and potential therapeutic interventions. This kit is a valuable asset for those studying mouse models of disease and seeking to better understand the molecular mechanisms underlying fibronectin-related pathologies.
Product Name:
Mouse FN (Fibronectin) CLIA Kit
SKU:
MOES00267
Target:
Mouse FN (Fibronectin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse FN. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse FN, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse FN. You can calculate the concentration of Mouse FN in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-98
98-112
88-103
Average (%)
93
103
95
1:4
Range (%)
100-118
94-108
86-99
Average (%)
108
100
93
1:8
Range (%)
83-98
98-114
86-99
Average (%)
90
105
92
1:16
Range (%)
86-99
100-115
88-101
Average (%)
91
107
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-106
98
EDTA plasma (n=5)
97-110
103
Cell culture media (n=5)
97-111
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.09
2.6
9.05
1.08
2.58
8.64
Standard deviation
0.11
0.18
0.8
0.13
0.24
0.55
C V (%)
10.09
6.92
8.84
12.04
9.3
6.37
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse FN concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse FN in samples. No significant cross-reactivity or interference between Mouse FN and analogues was observed.
