The Mouse GGT1 (Gamma-Glutamyltransferase 1) ELISA Kit is specifically designed for the precise quantification of GGT1 levels in mouse serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, ensuring accurate and reproducible results for various research applications.GGT1 is an important enzyme that plays a vital role in the detoxification process and is involved in the metabolism of glutathione. It is also associated with liver function and certain diseases such as liver cancer, liver cirrhosis, and alcoholic liver disease.
Monitoring GGT1 levels can provide valuable insights into these conditions and aid in the development of potential treatments.Overall, the Mouse GGT1 ELISA Kit is a valuable tool for researchers studying liver function, metabolism, and related diseases in mouse models. Its high performance and reliability make it an essential component of any laboratory conducting research in this area.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse gGT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse gGT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse gGT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse gGT1. You can calculate the concentration of Mouse gGT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-105
89-103
86-100
Average (%)
96
94
92
1:4
Range (%)
89-100
86-100
85-98
Average (%)
95
91
90
1:8
Range (%)
93-106
81-94
88-100
Average (%)
99
88
94
1:16
Range (%)
89-102
80-92
83-95
Average (%)
94
85
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
87-102
93
EDTA plasma (n=5)
89-105
97
Cell culture media (n=5)
90-102
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.09
4.02
0.44
1.06
3.93
Standard deviation
0.03
0.05
0.15
0.02
0.05
0.21
C V (%)
6.25
4.59
3.73
4.55
4.72
5.34
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse gGT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse gGT1 in samples. No significant cross-reactivity or interference between Mouse gGT1 and analogues was observed.
