The Mouse GRN (Granulin) ELISA Kit is specifically designed for the precise measurement of granulin levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it perfect for a variety of research purposes.Granulin is a key protein that plays a vital role in various physiological processes, including cell growth, inflammation, and tissue repair. Dysregulation of granulin expression has been linked to multiple diseases, such as cancer, Alzheimer's, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential treatments. Overall, the Mouse GRN ELISA Kit from Assay Genie provides researchers with a powerful tool to investigate the role of granulin in health and disease, offering reliable data that can contribute to advancing scientific knowledge and therapeutic strategies.
Product Name:
Mouse GRN (Granulin) ELISA Kit
SKU:
MOES01100
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
