The Mouse HNF6a (Hepatocyte Nuclear Factor 6 Alpha) CLIA Kit is a specialized assay kit designed for the accurate detection of HNF6a levels in mouse serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and consistent results, making it an invaluable tool for various research applications.HNF6a is a key transcription factor involved in regulating liver development and function, as well as other important physiological processes.
Dysregulation of HNF6a has been linked to various liver disorders, making it a crucial biomarker for studying liver diseases and potential therapeutic interventions.With the Mouse HNF6a CLIA Kit, researchers can confidently measure HNF6a levels in biological samples with ease and efficiency, facilitating further insights into the molecular mechanisms underlying liver biology and disease progression.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse HNF6a. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HNF6a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HNF6a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse HNF6a. You can calculate the concentration of Mouse HNF6a in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-106
86-100
102-117
Average (%)
98
93
109
1:4
Range (%)
85-100
102-116
85-96
Average (%)
91
109
90
1:8
Range (%)
93-106
90-103
87-103
Average (%)
99
95
95
1:16
Range (%)
87-101
85-96
95-111
Average (%)
93
90
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-103
98
EDTA plasma (n=5)
87-97
92
Cell culture media (n=5)
97-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
50.21
103.14
380.42
46.36
103.69
415.6
Standard deviation
5.5
11.38
43.44
3.88
8.98
25.93
C V (%)
10.95
11.03
11.42
8.37
8.66
6.24
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse HNF6a concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse HNF6a in samples. No significant cross-reactivity or interference between Mouse HNF6a and analogues was observed.
