The Mouse IFN-Beta (Interferon-Beta) ELISA Kit is a specialized assay designed for the quantitative detection of interferon-beta levels in mouse serum, plasma, and tissue culture supernatants. This kit provides high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes. Interferon-beta is a key cytokine involved in regulating the immune response, particularly in antiviral defense and inflammatory pathways. Dysregulation of interferon-beta signaling has been implicated in various autoimmune disorders, viral infections, and inflammatory diseases, making it a crucial target for therapeutic interventions. With the Mouse IFN-Beta ELISA Kit, researchers can accurately measure interferon-beta levels in biological samples, enabling a better understanding of immune responses and potential therapeutic strategies for immune-related diseases. Its reliable performance and ease of use make it a valuable tool for studying the role of interferon-beta in health and disease.
Product Name:
Mouse IFN- beta (Interferon Beta) ELISA Kit
SKU:
MOES00653
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
