The Mouse IgG2a Immunoglobulin G2a CLIA Kit is specifically designed for the precise and accurate quantification of IgG2a levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.IgG2a is a key immunoglobulin subclass involved in immune response, inflammation, and antibody-mediated defense against pathogens.
It plays a crucial role in autoimmune diseases, infectious diseases, and vaccine development, making it a valuable biomarker for studying immune responses and developing targeted therapies.The Mouse IgG2a Immunoglobulin G2a CLIA Kit is a valuable tool for researchers looking to investigate immune responses and antibody production in mouse models, providing reliable and consistent results for their studies.
Product Name:
Mouse IgG2a (Immunoglobulin G2a) CLIA Kit
SKU:
MOES00379
Target:
Mouse IgG2a (Immunoglobulin G2a)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IgG2a. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IgG2a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IgG2a, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IgG2a. You can calculate the concentration of Mouse IgG2a in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
88-101
90-103
Average (%)
102
94
97
1:4
Range (%)
103-116
101-119
92-104
Average (%)
109
108
97
1:8
Range (%)
87-103
93-108
88-101
Average (%)
94
99
94
1:16
Range (%)
92-104
96-111
94-107
Average (%)
97
104
101
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-109
100
EDTA plasma (n=5)
91-105
96
Cell culture media (n=5)
101-115
106
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.58
14.98
41.52
4.55
14.38
40.61
Standard deviation
0.43
1.48
4.18
0.42
1.03
3.01
C V (%)
9.39
9.88
10.07
9.23
7.16
7.41
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IgG2a concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IgG2a in samples. No significant cross-reactivity or interference between Mouse IgG2a and analogues was observed.
