The Mouse IL-2 ELISA Kit is specifically designed for the precise measurement of interleukin-2 (IL-2) levels in mouse samples, such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for a variety of research applications.IL-2 is a key cytokine involved in regulating immune responses, playing a vital role in the proliferation and differentiation of T cells. Dysregulation of IL-2 has been implicated in autoimmune diseases, inflammation, and certain types of cancer, highlighting its importance as a biomarker in understanding these diseases and developing targeted therapies.Overall, the Mouse IL-2 ELISA Kit provides a reliable and efficient means of studying the role of IL-2 in immune function and disease pathogenesis, making it a valuable tool for researchers in the field.
Product Name:
Mouse IL-2 ELISA Kit
SKU:
MOES00661
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
