The Mouse IL-4 CLIA Kit is specifically designed for the quantitative measurement of IL-4 levels in mouse serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures accurate and reproducible results, making it an invaluable tool for a variety of research applications.IL-4 is a key cytokine involved in regulating immune responses and inflammation, playing a crucial role in conditions such as allergies, asthma, and autoimmune diseases.
By accurately measuring IL-4 levels, researchers can gain insights into the mechanisms underlying these diseases and identify potential therapeutic targets.Overall, the Mouse IL-4 CLIA Kit from Assay Genie provides researchers with a reliable and efficient solution for studying the role of IL-4 in immune regulation and disease pathogenesis.
Product Name:
Mouse IL-4 CLIA Kit
SKU:
MOES00040
Target:
Mouse IL-4
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
3.75 pg/mL
Detection range:
6.25-400 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse IL-4. You can calculate the concentration of Mouse IL-4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
97-110
86-102
Average (%)
103
104
93
1:4
Range (%)
97-112
91-101
98-114
Average (%)
105
96
107
1:8
Range (%)
85-97
91-104
84-96
Average (%)
91
98
91
1:16
Range (%)
91-103
97-113
99-115
Average (%)
97
103
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
99-114
105
EDTA plasma (n=5)
86-101
93
Cell culture media (n=5)
97-114
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
21.44
57.26
142.71
22.34
59.53
129.61
Standard deviation
2.57
4.57
12.47
2.48
5.64
13.74
C V (%)
11.99
7.98
8.74
11.1
9.47
10.6
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse IL-4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse IL-4 in samples. No significant cross-reactivity or interference between Mouse IL-4 and analogues was observed.
