The Mouse Insulin CLIA Kit is a highly sensitive and specific assay designed for the quantification of insulin levels in mouse serum, plasma, and tissue lysates. This kit provides reliable and reproducible results, making it ideal for a variety of research applications.Insulin is a key hormone involved in the regulation of glucose metabolism, energy storage, and cell growth. Dysregulation of insulin levels is associated with diseases such as diabetes, obesity, and metabolic syndrome, highlighting the importance of studying insulin levels in research settings.
By accurately measuring insulin levels in mouse samples, researchers can gain valuable insights into the role of insulin in various physiological and pathological processes. This kit is user-friendly and provides quick and accurate results, making it a valuable tool for investigating insulin signaling pathways and potential therapeutic interventions.
Product Name:
Mouse INS (Insulin) CLIA Kit
SKU:
MOES00049
Target:
Mouse INS (Insulin)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse INS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse INS. You can calculate the concentration of Mouse INS in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-108
102-115
99-115
Average (%)
101
108
107
1:4
Range (%)
87-102
100-117
84-99
Average (%)
93
108
91
1:8
Range (%)
84-98
103-117
101-112
Average (%)
90
109
106
1:16
Range (%)
94-108
90-103
84-95
Average (%)
99
97
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
99-115
107
Cell culture media (n=5)
97-113
103
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.98
4.54
19.56
2.06
4.87
18.18
Standard deviation
0.21
0.48
1.25
0.17
0.52
2.0
C V (%)
10.61
10.57
6.39
8.25
10.68
11.0
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse INS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse INS in samples. No significant cross-reactivity or interference between Mouse INS and analogues was observed.
