The Mouse INS (Insulin) ELISA Kit by Assay Genie is a specialized assay designed for the quantitative detection and measurement of Insulin levels in various biological samples collected from mouse models. Insulin is a pivotal hormone involved in the regulation of blood glucose levels, energy storage, metabolism, and various physiological processes. Accurate measurement of Insulin is crucial for studying glucose homeostasis, metabolic functions, and endocrine pathways related to insulin signaling in mouse research models. Our Mouse INS ELISA Kit ensures exceptional sensitivity and specificity, enabling reliable and reproducible results essential for investigating insulin dynamics, metabolic disorders, and potential therapeutic interventions. With stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for research studies focusing on elucidating the role of insulin in mouse physiology and pathogenesis. Trust Assay Genie's Mouse INS ELISA Kit for accurate and dependable quantification of Insulin levels in your mouse research studies.
Product Name:
Mouse INS (Insulin) ELISA Kit
SKU:
AEES00192
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
