The Mouse Klotho ELISA Kit is expertly designed to enable the precise quantitative measurement of Klotho levels in diverse biological samples, focusing on the characterization of this critical protein. Klotho, an essential anti-aging hormone, serves as a modulator of various cellular activities, impacting processes such as cell proliferation, differentiation, and apoptosis. With a pivotal role in regulating aging processes and maintaining homeostasis, Klotho emerges as a vital factor in promoting longevity and attenuating age-related disorders. Accurate determination of Klotho levels is paramount for elucidating its intricate functions in modulating aging-related pathways, including those involved in metabolic disorders, cardiovascular diseases, and renal function. By quantifying Klotho concentrations, researchers can gain crucial insights into its impact on cellular mechanisms, tissue homeostasis, and overall healthspan, providing a deeper understanding of how Klotho influences longevity and age-associated diseases. The Mouse Klotho ELISA Kit from Assay Genie guarantees exceptional sensitivity and specificity, ensuring dependable and reproducible results for your investigative needs. Manufactured under rigorous quality control standards, this kit delivers robust performance and user-friendly protocols, making it a preferred solution for research studies aiming to uncover the complex roles of Klotho in aging, health, and disease. Opt for Assay Genie's Klotho ELISA Kit to reliably quantify Klotho levels and advance your exploration of the science behind healthy aging and longevity.
Product Name:
Mouse Klotho (Klotho) ELISA Kit
SKU:
AEES00244
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
