Mouse LDLR (Low Density Lipoprotein Receptor) ELISA Kit
The Mouse LDLR (Low Density Lipoprotein Receptor) ELISA Kit is specifically designed for the accurate detection of LDLR levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.The low-density lipoprotein receptor (LDLR) is a critical protein involved in the regulation of cholesterol levels in the body, playing a key role in the uptake of LDL cholesterol from the bloodstream. Dysregulation of LDLR can lead to conditions such as hypercholesterolemia and atherosclerosis, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.With easy-to-follow instructions and all necessary components included, the Mouse LDLR ELISA Kit from Assay Genie is a valuable tool for researchers investigating the role of LDLR in cardiovascular diseases and lipid metabolism. Order now and take your research to the next level.
Product Name:
Mouse LDLR (Low Density Lipoprotein Receptor) ELISA Kit
SKU:
MOES01357
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.