The Mouse LEP (Leptin) ELISA Kit is a specialized assay designed for the precise quantitative measurement of leptin levels in various biological samples derived from mouse models. Leptin is a crucial hormone known for its role in regulating energy balance, metabolism, and adipose tissue development. It serves as a key factor in appetite control, energy expenditure, and the maintenance of body weight. With Assay Genie's Mouse LEP ELISA Kit, researchers can achieve reliable and reproducible results due to its exceptional sensitivity and specificity.
This kit offers a high-quality and efficient solution for studying leptin levels, providing valuable insights into metabolic functions, obesity-related mechanisms, and potential therapeutic strategies. Trust in the accuracy and performance of Assay Genie's Mouse LEP ELISA Kit for your research needs, ensuring a robust tool for investigating the intricate role of leptin in metabolic regulation and related physiological processes.
Product Name:
Mouse LEP (Leptin) ELISA Kit
SKU:
AEES00203
Target:
Mouse LEP (Leptin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse LEP. You can calculate the concentration of Mouse LEP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
97-108
89-104
Average (%)
91
104
96
1:4
Range (%)
84-93
85-98
92-103
Average (%)
90
90
97
1:8
Range (%)
91-100
93-99
98-107
Average (%)
96
96
103
1:16
Range (%)
87-98
82-97
83-97
Average (%)
94
90
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-107
99
EDTA plasma (n=5)
96-109
103
Cell culture media (n=5)
89-95
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.86
2.2
8.92
0.88
2.36
8.89
Standard deviation
0.04
0.1
0.48
0.05
0.11
0.4
C V (%)
4.65
4.55
5.38
5.68
4.66
4.5
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse LEP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse LEP in samples. No significant cross-reactivity or interference between Mouse LEP and analogues was observed.
