The Mouse NOS3 (eNOS) Nitric Oxide Synthase 3 Endothelial ELISA Kit is specifically designed for the accurate measurement of nitric oxide synthase 3 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Nitric oxide synthase 3, also known as endothelial nitric oxide synthase (eNOS), is a key enzyme involved in the production of nitric oxide, a critical signaling molecule in various physiological processes. Dysregulation of eNOS has been implicated in conditions such as cardiovascular diseases, diabetes, and inflammation, highlighting its importance as a potential biomarker for studying and treating these diseases.
With the Mouse NOS3 (eNOS) Nitric Oxide Synthase 3 Endothelial ELISA Kit, researchers can accurately quantify eNOS levels in mouse samples, facilitating further understanding of its role in health and disease. This kit is a valuable tool for investigating the mechanisms underlying eNOS-mediated pathways and exploring potential therapeutic interventions.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse NOS3/eNOS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse NOS3/eNOS. You can calculate the concentration of Mouse NOS3/eNOS in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-110
94-110
86-102
Average (%)
102
101
93
1:4
Range (%)
98-113
82-96
97-114
Average (%)
105
88
104
1:8
Range (%)
95-109
84-97
98-111
Average (%)
103
91
103
1:16
Range (%)
96-109
82-94
94-107
Average (%)
103
88
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
90-104
97
Cell culture media (n=5)
92-104
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
45.5
109.2
426.3
44.3
119.4
394.8
Standard deviation
3.2
5.1
17.9
2.4
6.2
18.6
C V (%)
7.03
4.67
4.2
5.42
5.19
4.71
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse NOS3/eNOS concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NOS3/eNOS in samples. No significant cross-reactivity or interference between Mouse NOS3/eNOS and analogues was observed.
