The Mouse NT-proBNP (N-Terminal Pro-brain Natriuretic Peptide) CLIA Kit is a highly sensitive and specific assay designed for the quantification of NT-proBNP levels in mouse serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it ideal for various research applications related to cardiovascular health and disease.NT-proBNP is a biomarker that is commonly used in the diagnosis and prognosis of heart failure, as elevated levels are indicative of cardiac stress and dysfunction.
Monitoring NT-proBNP levels can help researchers better understand the progression of heart disease and evaluate the effectiveness of potential interventions.With its high performance and accuracy, the Mouse NT-proBNP CLIA Kit is essential for studying cardiovascular conditions in mouse models and developing new therapeutic strategies to improve heart health.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse NT-proBNP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse NT-proBNP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Mouse NT-proBNP. You can calculate the concentration of Mouse NT-proBNP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-113
88-101
86-97
Average (%)
107
95
92
1:4
Range (%)
88-100
98-114
90-101
Average (%)
93
104
96
1:8
Range (%)
88-102
99-114
86-98
Average (%)
93
105
91
1:16
Range (%)
98-116
96-110
97-113
Average (%)
106
103
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
101
EDTA plasma (n=5)
91-104
96
Cell culture media (n=5)
88-101
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
23.67
58.85
245.1
25.59
53.51
264.09
Standard deviation
2.45
6.99
16.32
2.88
6.19
25.27
C V (%)
10.35
11.88
6.66
11.25
11.57
9.57
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Mouse NT-proBNP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse NT-proBNP in samples. No significant cross-reactivity or interference between Mouse NT-proBNP and analogues was observed.
