The Mouse OXA (Orexin A) ELISA Kit is specifically designed for the precise measurement of orexin A levels in mouse serum, plasma, and tissue homogenates. With its exceptional sensitivity and specificity, this kit delivers consistent and accurate results, making it a valuable tool for various research endeavors.Orexin A is a neuropeptide that plays a crucial role in regulating wakefulness and feeding behavior.
Dysregulation of orexin A has been implicated in various disorders, including sleep disorders, obesity, and addiction. Therefore, monitoring orexin A levels can provide valuable insights into these conditions and aid in the development of new therapeutic strategies.Get your Mouse OXA ELISA Kit now and unlock the potential for groundbreaking discoveries in the field of neuroscience and metabolism research.
Product Name:
Mouse OXA (Orexin A) ELISA Kit
SKU:
MOES01327
Target:
Mouse OXA (Orexin A)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with OXA. During the reaction, OXA in the sample or standard competes with a fixed amount of OXA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to OXA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of OXA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-103
85-96
99-115
Average (%)
94
91
105
1:4
Range (%)
84-97
93-108
95-110
Average (%)
91
100
101
1:8
Range (%)
87-98
91-106
100-115
Average (%)
93
98
107
1:16
Range (%)
90-104
91-102
94-106
Average (%)
97
96
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
88-99
93
Cell culture media (n=5)
93-104
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
197.4
441.8
1403.3
188.1
428.5
1373.9
Standard deviation
12.6
19.9
60.3
10.0
23.6
52.2
C V (%)
6.38
4.5
4.3
5.32
5.51
3.8
Sample type &Sample volume:
Serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of OXA concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes OXA in samples. No significant cross-reactivity or interference between OXA and analogues was observed.
