The Mouse POSTN (OSF-2, Periostin) ELISA Kit is specifically designed for the quantitative detection of POSTN levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.POSTN, also known as OSF-2 or Periostin, is a key protein involved in tissue remodeling and repair processes, playing a critical role in cell adhesion, migration, and proliferation.
Its dysregulation has been implicated in various diseases, including cancer, cardiovascular diseases, and skeletal disorders, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the Mouse POSTN (OSF-2, Periostin) ELISA Kit provides researchers with a reliable tool to investigate the role of POSTN in disease pathogenesis and to identify potential targets for therapeutic interventions.
Product Name:
Mouse POSTN/OSF-2 (Periostin) ELISA Kit
SKU:
MOES01141
Target:
Mouse POSTN/OSF-2 (Periostin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
4.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse POSTN/OSF-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse POSTN/OSF-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse POSTN/OSF-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse POSTN/OSF-2. You can calculate the concentration of Mouse POSTN/OSF-2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-100
100-112
97-109
Average (%)
91
106
103
1:4
Range (%)
92-110
84-95
82-96
Average (%)
100
90
88
1:8
Range (%)
88-101
84-96
83-94
Average (%)
94
90
88
1:16
Range (%)
88-99
87-99
87-98
Average (%)
93
93
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-105
99
EDTA plasma (n=5)
87-101
94
Cell culture media (n=5)
85-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.5
1.41
4.38
0.49
1.28
4.53
Standard deviation
0.03
0.08
0.16
0.03
0.07
0.22
C V (%)
6
5.67
3.65
6.12
5.47
4.86
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse POSTN/OSF-2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse POSTN/OSF-2 in samples. No significant cross-reactivity or interference between Mouse POSTN/OSF-2 and analogues was observed.
