The Mouse PTGS2 (COX-2) Prostaglandin Endoperoxide Synthase 2 ELISA Kit is specifically designed for the accurate measurement of PTGS2 levels in mouse biological samples such as serum, plasma, and tissue lysates. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.PTGS2, also known as cyclooxygenase-2 (COX-2), is an important enzyme involved in the production of prostaglandins, which play a crucial role in inflammation and pain. Dysregulation of PTGS2 has been implicated in various diseases such as cancer, cardiovascular diseases, and inflammatory disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.With its reliable performance and ease of use, the Mouse PTGS2 (COX-2) Prostaglandin Endoperoxide Synthase 2 ELISA Kit is an essential tool for researchers looking to investigate the role of PTGS2 in disease pathogenesis and therapeutic targeting.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
