SynonymsM2PK,PKM,M2-PK,PKM1,CTHBP,OIP3,PK3,PKM,TCB,THBP1,Pyruvate kinase muscle isozyme,Thyroid hormone-binding protein 1,Cytosolic thyroid hormone-binding protein
ReactivityMouse
Sample TypeSerum, Plasma And Other Biological Fluids
SKU:
MODL01055
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P52480
Sensitivity:
0.144ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
M2PK,PKM,M2-PK,PKM1,CTHBP,OIP3,PK3,PKM,TCB,THBP1,Pyruvate kinase muscle isozyme,Thyroid hormone-binding protein 1,Cytosolic thyroid hormone-binding protein
The Mouse Pyruvate Kinase Muscle (PKM2) ELISA Kit is specifically designed for accurate detection of PKM2 levels in mouse serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.PKM2 is a key enzyme involved in glycolysis and is known to play a crucial role in regulating cellular metabolism and energy production.
Dysregulation of PKM2 has been associated with various diseases, including cancer and metabolic disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.With its user-friendly protocol and robust performance, the Mouse Pyruvate Kinase Muscle (PKM2) ELISA Kit is an essential tool for researchers investigating the role of PKM2 in health and disease.
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
