The Mouse REN (Renin) ELISA Kit is specifically designed for the precise measurement of renin levels in mouse serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.Renin is a key enzyme in the renin-angiotensin system, playing a critical role in regulating blood pressure and electrolyte balance. Dysregulation of renin activity is associated with hypertension, kidney diseases, and other cardiovascular conditions, making it an important target for therapeutic interventions. By utilizing the Mouse REN ELISA Kit, researchers can effectively study the role of renin in physiological and pathological processes, leading to a better understanding of cardiovascular health and the development of potential treatment strategies.
Product Name:
Mouse REN (Renin) ELISA Kit
SKU:
MOES00673
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
