The Mouse TJP1 (Tight Junction Protein 1) ELISA Kit is specifically designed for the precise measurement of tight junction protein 1 levels in mouse serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it perfect for various research applications.Tight junction protein 1 is a key component of tight junctions, which are crucial for maintaining the integrity of epithelial and endothelial cell barriers. Dysregulation of TJP1 has been linked to various diseases, including inflammatory bowel disease, liver diseases, and cancer. Therefore, this ELISA kit is a valuable tool for studying the role of TJP1 in these conditions and exploring potential therapeutic interventions.Order the Mouse TJP1 ELISA Kit today and unlock the potential of your research in understanding tight junction biology and its implications in health and disease. Visit the website [here](www.assaygenie.com/mouse-tjp1-tight-junction-protein-1-elisa-kit-moes01563/) to learn more and make your purchase.
Product Name:
Mouse TJP1 (Tight Junction Protein 1) ELISA Kit
SKU:
MOES01563
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
