The Mouse TLR4 (Toll-like Receptor 4) ELISA Kit is specifically designed for the accurate measurement of TLR4 levels in mouse serum, plasma, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring reliable and consistent results for a variety of research applications.TLR4 is a key receptor in the innate immune system, playing a crucial role in detecting and responding to bacterial lipopolysaccharides. Dysregulation of TLR4 signaling has been implicated in various inflammatory diseases, making it a valuable biomarker for studying immune responses and developing targeted therapies.
By utilizing the Mouse TLR4 ELISA Kit, researchers can accurately quantify TLR4 levels in biological samples, allowing for in-depth analysis of immune system function and potential therapeutic interventions. Trust in the precision and reliability of this kit to advance your research in immunology and inflammation-related studies.
Product Name:
Mouse TLR4 (Toll-Like Receptor 4) ELISA Kit
SKU:
MOES01697
Target:
Mouse TLR4 (Toll-Like Receptor 4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TLR4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TLR4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TLR4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TLR4. You can calculate the concentration of Mouse TLR4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
91-106
95-112
Average (%)
103
98
103
1:4
Range (%)
89-103
87-98
87-99
Average (%)
95
93
94
1:8
Range (%)
90-102
82-94
84-99
Average (%)
96
89
91
1:16
Range (%)
91-105
87-99
82-93
Average (%)
97
93
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
88-104
95
Cell culture media (n=5)
91-108
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.4
3.66
0.5
1.33
3.57
Standard deviation
0.03
0.07
0.2
0.03
0.08
0.13
C V (%)
6.52
5.0
5.46
6.0
6.02
3.64
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Mouse TLR4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Mouse TLR4 in samples. No significant cross-reactivity or interference between Mouse TLR4 and analogues was observed.
