The Mouse IL-1? ELISA Kit - High Sensitivity is a specialized assay developed for the accurate and sensitive quantification of Interleukin-1 alpha (IL-1?) levels in various mouse biological samples. IL-1? is a pivotal pro-inflammatory cytokine that participates in a diverse range of cellular processes, including cell growth, differentiation, and programmed cell death. This cytokine exerts critical functions in initiating and regulating immune responses and inflammatory reactions, contributing significantly to the body's defense mechanisms and the pathogenesis of various inflammatory conditions, autoimmune disorders, and infectious diseases. With its high sensitivity and specificity, the Mouse IL-1? ELISA Kit enables researchers to precisely measure IL-1? concentrations, providing vital insights into its roles in disease mechanisms and as a potential therapeutic target. Accurate quantification of IL-1? levels is crucial for elucidating the complex pathways involved in immune responses and inflammatory conditions, paving the way for the development of targeted therapeutic interventions. Backed by stringent quality control measures, this ELISA kit from Assay Genie offers robust performance, delivering reliable and reproducible results for research applications. Its user-friendly design and exceptional performance make it an invaluable tool for investigators studying inflammation, immunology, and disease pathogenesis. Get ready to uncover the intricate roles of IL-1? in biological processes with confidence and precision using the Mouse IL-1? ELISA Kit - High Sensitivity.
Product Name:
Mouse IL-1 alpha ELISA Kit (High Sensitivity)
SKU:
AEES00626
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
3.01 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
