The Mouse RETN (Resistin) ELISA Kit - High Sensitivity is a specialized assay designed for the precise quantitative measurement of Resistin levels in various biological samples from mouse subjects. Resistin, an essential adipocytokine, plays a crucial role in various metabolic pathways, inflammation, and immune responses, making it an indispensable biomarker for research into metabolic disorders, inflammation-related conditions, and immune system modulation. With a focus on high sensitivity, this ELISA kit offers researchers a powerful tool to investigate the intricate involvement of Resistin in metabolic functions, adipose tissue biology, and inflammatory processes with exceptional precision. The advanced sensitivity of this kit enables researchers to delve deeper into the role of Resistin in metabolic homeostasis and its implications for various physiological processes. The Mouse RETN ELISA Kit - High Sensitivity from Assay Genie provides excellent sensitivity and specificity, ensuring reliable and reproducible results. Manufactured under strict quality control measures, this kit delivers robust performance and ease of use, making it an optimal choice for research exploring the complex interplay of Resistin in metabolic and immunological pathways in mouse models.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
