The MYOT Antibody (CAB6439) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. RRID AB_2767041 Gene ID 9499 Swiss Prot Synonym MFM3; TTID; TTOD; LGMD1; LGMD1A; MYOT
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
MYOT Antibody
SKU:
CAB6439
Size:
100μL, 20μL
Reactivity:
Human, Mouse, Rat
Clone Number:
-
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IF
/
ICC
1:10 - 1:100
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
MFM3, TTID, TTOD, LGMD1, LGMD1A, MYOT
Positive Sample:
293T, HepG2, Mouse skeletal muscle, Mouse heart, Rat skeletal muscle, Rat heart
Cellular Localization:
Cell Membrane, Cytoplasm, Z Line, Cytoskeleton, Myofibril, Sarcolemma, Sarcomere.
Calculated MW:
55kDa
Observed MW:
55kDa
This gene encodes a cystoskeletal protein which plays a significant role in the stability of thin filaments during muscle contraction. This protein binds F-actin, crosslinks actin filaments, and prevents latrunculin A-induced filament disassembly. Mutations in this gene have been associated with limb-girdle muscular dystrophy and myofibrillar myopathies. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. RRID AB_2767041 Gene ID 9499 Swiss Prot Synonym MFM3; TTID; TTOD; LGMD1; LGMD1A; MYOT
Purification Method:
Affinity purification
Gene ID:
9499
RRID:
AB_2767041
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using MYOT Rabbit pAb (CAB6439) at 1:1000 dilution._Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using MYOT Rabbit pAb (CAB6439). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
