The NHEJ1 Polyclonal Antibody (CAB21661) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to NHEJ1 protein and is validated for use in Western blot applications. It enables detection and analysis of NHEJ1 in various cell types, providing valuable insights into the role of this protein in DNA double-strand break repair.NHEJ1, also known as non-homologous end joining protein 1, is an essential component of the non-homologous end joining pathway, a key mechanism for repairing DNA double-strand breaks.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
NHEJ1 Polyclonal Antibody
SKU:
CAB21661
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
XLF, NHEJ1
Cellular Localization:
Nucleus.
Calculated MW:
33kDa
Observed MW:
Refertofigures
Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders.
Purification Method
Affinity purification
Gene ID
79840
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using NHEJ1 Rabbit pAb (CAB21661) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 5s.
