The TIAL1 Antibody (CAB6075) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity for human samples and has been validated for Western blot applications. By binding to the nucleolysin TIAR protein, researchers can easily detect and analyze this protein in various cell types, making it an ideal choice for studies in molecular biology and cellular stress response mechanisms.Nucleolysin TIAR, also known as TIA-1-related protein, is involved in the regulation of mRNA translation, RNA splicing, and stress granule formation, making it a key player in cellular response to environmental stressors and cellular signaling pathways.
This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
TIAL1 Antibody
SKU:
CAB6075
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
TCBP, TIAR, TIAL1
Positive Sample:
A-549, HeLa, K-562
Cellular Localization:
Cytoplasm, Cytoplasmic Granule, Nucleus.
Calculated MW:
42kDa
Observed MW:
47kDa
The protein encoded by this gene is a member of a family of RNA-binding proteins, has three RNA recognition motifs (RRMs), and binds adenine and uridine-rich elements in mRNA and pre-mRNAs of a wide range of genes. It regulates various activities including translational control, splicing and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The different isoforms have been show to function differently with respect to post-transcriptional silencing.
Purification Method
Affinity purification
Gene ID
7073
RRID
AB_2766738
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using TIAL1 Rabbit pAb (CAB6075) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat lung using TIAL1 Rabbit pAb (CAB6075) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using TIAL1 Rabbit pAb (CAB6075) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
