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p53 Polyclonal Antibody (CAB21631)

SKU:
CAB21631
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
IHC
IP
€129 - €359
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:p53 Polyclonal Antibody
SKU:CAB21631
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-393 of human p53 (NP_000537.3).
Sequence:MEEP QSDP SVEP PLSQ ETFS DLWK LLPE NNVL SPLP SQAM DDLM LSPD DIEQ WFTE DPGP DEAP RMPE AAPP VAPA PAAP TPAA PAPA PSWP LSSS VPSQ KTYQ GSYG FRLG FLHS GTAK SVTC TYSP ALNK MFCQ LAKT CPVQ LWVD STPP PGTR VRAM AIYK QSQH MTEV VRRC PHHE RCSD SDGL APPQ HLIR VEGN LRVE YLDD RNTF RHSV VVPY EPPE VGSD CTTI HYNY MCNS SCMG GMNR RPIL TIIT LEDS SGNL LGRN SFEV RVCA CPGR DRRT EEEN LRKK GEPH HELP PGST KRAL PNNT SSSP QPKK KPLD GEYF TLQI RGRE RFEM FREL NEAL ELKD AQAG KEPG GSRA HSSH LKSK KGQS TSRH KKLM FKTE GPDS D
Tested Applications:WB IHC-P IP ELISA
Recommended Dilution:WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:P53; BCC7; LFS1; BMFS5; TRP53; p53
Positive Sample:293T,NIH/3T3,Rat lung
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body.
Calculated MW:44kDa
Observed MW:Refer to figures

This gene encodes a tumor suppressor protein containing transcriptional activation, DNA binding, and oligomerization domains. The encoded protein responds to diverse cellular stresses to regulate expression of target genes, thereby inducing cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. Mutations in this gene are associated with a variety of human cancers, including hereditary cancers such as Li-Fraumeni syndrome. Alternative splicing of this gene and the use of alternate promoters result in multiple transcript variants and isoforms. Additional isoforms have also been shown to result from the use of alternate translation initiation codons from identical transcript variants (PMIDs: 12032546, 20937277).

Purification Method:Affinity purification
Gene ID:7157
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using p53 Rabbit pAb (CAB21631) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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