The PDLIM5 Polyclonal Antibody (CAB22013) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.PDLIM5, also known as Enigma homolog protein, is a cytoskeletal protein that plays a role in cell adhesion, migration, and signaling. Its involvement in these processes makes it a key protein of interest in studies focusing on cell biology, cancer research, and developmental biology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
PDLIM5 Polyclonal Antibody
SKU:
CAB22013
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
L9, ENH, LIM, ENH1, PDLIM5
Positive Sample:
HeLa
Cellular Localization:
Actin Cytoskeleton, Cell Projection, Cytosol, Postsynaptic Density, Presynapse, Z Disc.
Calculated MW:
64kDa
Observed MW:
64kDa
This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
10611
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using PDLIM5 Rabbit pAb (CAB22013) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
