The Phospho-AMPKalpha1-S496 Monoclonal Antibody (CABP1002) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high sensitivity and specificity towards human samples and is validated for use in Western blot applications. AMP-activated protein kinase (AMPK) is a central player in cellular energy homeostasis, regulating processes such as glucose uptake and fatty acid oxidation. The phosphorylation of AMPK at serine 496 (S496) is known to modulate its activity, impacting downstream signaling cascades involved in metabolism, cell growth, and autophagy.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-AMPKalpha1-S496 Monoclonal Antibody
SKU:
CABP1002
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC1547
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
SGSV S
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AMPK, AMPKa1, AMPK alpha 1, Phospho-AMPKα1-S496
Positive Sample:
C2C12 treated with oligomycin
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
64kDa
Observed MW:
64kDa
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Purification Method
Affinity purification
Gene ID
5562
RRID
AB_2863894
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKα1-S496 Rabbit mAb (CABP1002) at 1:1000 dilution. C2C12 cells were treated with oligomycin (0. 5 μM) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
