The Phospho-Histone H3-S10 Antibody (CABP0840) is a high-quality antibody developed for reliable detection and analysis of target proteins. Histone H3 is a key protein involved in chromatin structure and regulation of gene expression, and phosphorylation at Ser10 is known to play a role in cell cycle progression and mitosis.This antibody, raised in rabbits, is highly reactive with human and other mammalian samples, and has been validated for use in Western blot and immunohistochemistry applications. It specifically recognizes the phosphorylated form of Histone H3 at Ser10, allowing for detection and analysis of this critical post-translational modification in various cell types and tissues.The phosphorylation of Histone H3 at Ser10 has been implicated in various biological processes, including chromatin condensation, gene transcription, and DNA repair.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Phospho-Histone H3-S10 Antibody
SKU:
CABP0840
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ARKS T
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:1000 - 1:5000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa treated with Nocodazole, NIH/3T3 treated with Nocodazole, C6 treated with Nocodazole
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
15kDa
Observed MW:
17kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method
Affinity purification
Gene ID
8290 8350
RRID
AB_2771171
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-Histone H3-S10 Rabbit pAb (CABP0840) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated with Nocodazole (50 ng/ml) at 37℃ for 20 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-Histone H3-S10 Rabbit pAb (CABP0840) at dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10 Rabbit pAb (CABP0840) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10 Rabbit pAb (CABP0840) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
