The Phospho-KAP1/TRIM28-S824 Monoclonal Antibody (CABP1393) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-KAP1/TRIM28-S824 Monoclonal Antibody
SKU:
CABP1393
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
ARC59356
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:1000 - 1:5000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
HeLa treated with Etoposide, NIH/3T3 treated with Etoposide
Cellular Localization:
Nucleus.
Calculated MW:
79kDa/89kDa
Observed MW:
117kDa
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
Purification Method
Affinity purification
Gene ID
10155
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-KAP1/TRIM28-S824 Rabbit mAb (CABP1393) at 1:5000 dilution. HeLa cells were treated with Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-KAP1/TRIM28-S824 Rabbit mAb (CABP1393) at 1:5000 dilution. NIH/3T3 cells were treated with Etoposide (25 uM) at 37℃ for 5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 60s.
