The Phospho-p70 S6 Kinase 1-T421/S424 Antibody (CABP1106) is a high-quality antibody developed for reliable detection and analysis of target proteins. This rabbit polyclonal antibody is highly reactive with samples from multiple species, including human, mouse, and rat, and has been validated for use in Western blot and immunohistochemistry applications.The antibody binds to the phosphorylated form of p70 S6 kinase at threonine 421 and serine 424, allowing for precise detection and analysis of this critical signaling pathway component in various cell types. It is an essential tool for studying the regulation of cell proliferation, differentiation, and survival, making it particularly useful for research in cancer biology and drug development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-p70 S6 Kinase 1-T421/S424 Antibody
SKU:
CABP1106
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
RTPV SP
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a member of the ribosomal S6 kinase family of serine/threonine kinases. The encoded protein responds to mTOR (mammalian target of rapamycin) signaling to promote protein synthesis, cell growth, and cell proliferation. Activity of this gene has been associated with human cancer. Alternatively spliced transcript variants have been observed. The use of alternative translation start sites results in isoforms with longer or shorter N-termini which may differ in their subcellular localizations. There are two pseudogenes for this gene on chromosome 17.
Purification Method
Affinity purification
Gene ID
6198
RRID
AB_2863975
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p70 S6 Kinase 1-T421/S424 pAb (CABP1106) at 1:1000 dilution or P70 S6K antibody (CAB16968). NIH/3T3 cells were treated with EGF (100 ng/mL) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
