The Phospho-PPP2CA/PPP2CB-Y307 Antibody (CABP0927) is a high-quality antibody developed for reliable detection and analysis of target proteins. This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008] RRID AB_2863839 Gene ID 5515 5516 Swiss Prot Synonym PPP2CA/PPP2CB; Phospho-PPP2CA/PPP2CB-Y307
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
Phospho-PPP2CA/PPP2CB-Y307 Antibody
SKU:
CABP0927
Size:
100μL, 20μL
Reactivity:
Human, Mouse
Clone Number:
-
Conjugate:
-
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PPP2CA/PPP2CB, Phospho-PPP2CA/PPP2CB-Y307
Positive Sample:
HeLa treated with Calyculin A, NIH/3T3 treated with Calyculin A
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008] RRID AB_2863839 Gene ID 5515 5516 Swiss Prot Synonym PPP2CA/PPP2CB; Phospho-PPP2CA/PPP2CB-Y307
Purification Method:
Affinity purification
Gene ID:
5515 5516
RRID:
AB_2863839
Buffer Information:
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-PPP2CA/PPP2CB-Y307 Rabbit pAb (CABP0927) at 1:1000 dilution. HeLa cells were treated with Calyculin A (100nM) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated with Calyculin A (100nM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
