The Phospho-PRKDC-S2056 Antibody (CABP0621) is a high-quality antibody developed for reliable detection and analysis of target proteins. PRKDC is a critical player in DNA damage response and repair processes, making it a crucial target for cancer research and therapy development.This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting phosphorylation at S2056 of PRKDC in human samples. It has been rigorously validated for use in Western blot applications, allowing researchers to accurately assess PRKDC phosphorylation levels in various cell types and conditions.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-PRKDC-S2056 Antibody
SKU:
CABP0621
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
YSSQ DP
Tested Applications:
WBIHC-PIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:2000
IHC-P
1:100 - 1:500
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.
Purification Method
Affinity purification
Gene ID
5591
RRID
AB_2771465
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-DNA-PKcs-S2056 Rabbit pAb (CABP0621). Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
Immunohistochemistry analysis of paraffin-embedded Human small intestine tissue using Phospho-DNA-PKcs-S2056 Rabbit pAb (CABP0621) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
