The Phospho-RELB-S573 Polyclonal Antibody (CABP1297) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically binds to the phosphorylated form of RelB at serine 573, allowing for the detection and analysis of this post-translational modification in various cell types.RelB is a key player in the NF-κB signaling pathway, which is known to be dysregulated in various diseases including cancer, autoimmune disorders, and chronic inflammatory conditions. Understanding the phosphorylation status of RelB at S573 can provide insights into the regulation of NF-κB activity and its role in disease pathogenesis.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-RELB-S573 Polyclonal Antibody
SKU:
CABP1297
Size:
20μL, 100μL
Reactivity:
Mouse
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
LLSP G
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Enables RNA polymerase II cis-regulatory region sequence-specific DNA binding activity and protein kinase binding activity. Involved in lymphocyte differentiation and negative regulation of interferon-beta production. Located in cytosol and nucleoplasm. Part of chromatin; nucleus; and transcription repressor complex. Colocalizes with centrosome. Implicated in breast cancer and immunodeficiency 53. Biomarker of breast cancer and transitional cell carcinoma.
Purification Method
Affinity purification
Gene ID
5971
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A20, using Phospho-RELB-S573 Rabbit pAb (CABP1297) at 1:500 dilution. A20 cells were treated with PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
