The Porcine Interleukin-1 Beta (IL1B) ELISA Kit is a powerful tool for the precise measurement of IL1B levels in porcine samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for various research endeavors.IL1B is a key cytokine involved in inflammation and immune responses, playing a vital role in various physiological processes and disease pathways. By accurately quantifying IL1B levels, researchers can gain crucial insights into inflammatory conditions, infectious diseases, and autoimmune disorders, paving the way for advancements in diagnostics and therapeutics.
The Porcine IL1B ELISA Kit from Assay Genie provides researchers with a reliable and efficient platform to explore the intricate mechanisms of IL1B signaling and its implications in health and disease. With its user-friendly protocol and robust performance, this kit is a must-have for investigators seeking to unravel the complexities of porcine IL1B biology. Order now and elevate your research to new heights.
Product Name:
Porcine Interleukin 1 Beta (IL-1b) ELISA Kit
Product Code:
PRDL00089
Size:
96 Assays
Target:
IL1b
Synonyms:
IL1-B, IL1-Beta, IL1F2, IL-1?, Interleukin-1 Family Member 2, Catabolin
Detection Method:
Sandwich
Range:
62.5-4000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
