The PP2A-B56delta/PR61delta/PPP2R5E Polyclonal Antibody (CAB19333) is a high-quality antibody developed for reliable detection and analysis of target proteins. PPP2R5E (PP2A-B56ε) is a regulatory subunit of Protein Phosphatase 2A (PP2A), one of the four major serine/threonine phosphatases implicated in the negative control of cell growth and division. It belongs to the B56 subfamily and modulates the substrate selectivity and catalytic activity of the PP2A holoenzyme by associating with its core catalytic and regulatory subunits. Dysregulation of PP2A signaling has been linked to tumorigenesis and various cellular dysfunctions, making this regulatory subunit of significant interest in cancer and cell cycle research.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Rat samples.
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
B56E, B56epsilon, PP2A-B56δ/PR61δ/PPP2R5E
Positive Sample:
Rat spleen
Cellular Localization:
Cytoplasm.
Calculated MW:
55kDa
Observed MW:
49kDa
The protein encoded by this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. Multiple transcript variants encoding several different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
5529
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat spleen using PP2A-B56δ/PR61δ/PPP2R5E Rabbit pAb (CAB19333) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 15s.
