The PP2A-B56delta/PR61delta/PPP2R5D Antibody (CAB15707) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in various applications, including Western blotting. By binding to PPP2R5D, this antibody allows for the detection and analysis of this important protein in different cell types, making it an essential tool for studies in molecular biology and cell signaling pathways.PP2A is a critical enzyme involved in the regulation of numerous cellular processes, including cell growth, division, and differentiation.
This antibody is validated for use in WB, IHC-P, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
PP2A-B56delta/PR61delta/PPP2R5D Antibody
SKU:
CAB15707
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
B56D, MRD35, B56delta, PP2A-B56δ/PR61δ/PPP2R5D
Positive Sample:
HT-29, 293T, HeLa, Mouse heart, Mouse kidney, Rat kidney
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
70kDa
Observed MW:
80kDa
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a delta isoform of the regulatory subunit B56 subfamily. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method
Affinity purification
Gene ID
5528
RRID
AB_2763119
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using PP2A-B56δ/PR61δ/PPP2R5D Rabbit pAb (CAB15707) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
Immunohistochemistry analysis of paraffin-embedded Rat brain using PP2A-B56δ/PR61δ/PPP2R5D Rabbit pAb (CAB15707) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PP2A-B56δ/PR61δ/PPP2R5D Rabbit pAb (CAB15707) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of L929 cells using PP2A-B56δ/PR61δ/PPP2R5D Rabbit pAb (CAB15707) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
