The QuickStep Human Fe Ferritin ELISA Kit is specifically designed to accurately detect and measure levels of ferritin in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a variety of research applications.Ferritin is a key protein involved in iron storage and regulation in the body, making it a vital biomarker for conditions such as iron deficiency anemia, hemochromatosis, and other iron-related disorders.
Monitoring ferritin levels can help researchers better understand these conditions and develop targeted therapies.With the QuickStep Human Fe Ferritin ELISA Kit, researchers can confidently study the role of ferritin in various diseases and conditions, enabling advancements in diagnostics and treatment strategies.
Product Name:
QuickStep Human Ferritin ELISA Kit
Product Code:
QSES017
Assay Type:
Sandwich
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Human
Sensitivity:
24.31 pg/mL
Range:
62.50-4000 pg/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Ferritin
Application:
This ELISA kit applies to the in vitro quantitative determination of FE concentrations in serum and plasma.
Specificity:
This kit recognizes FE in samples. No significant cross-reactivity or interference between FE and analogues was observed.
Dilution Method:
It is recommended to dilute normal serum/plasma samples at 40-120 fold.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human FE. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human FE are added to the micro ELISA plate wells. Human FE in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human FE. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human FE. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (pg/mL)
183.00
362.63
1422.41
190.21
332.97
1312.84
Standard deviation
12.04
15.56
58.60
10.44
16.62
69.19
CV (%)
6.58
4.29
4.12
5.49
4.99
5.27
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
87-101
92
EDTA plasma(n=8)
90-105
97
Urine(n=8)
99
Samples were spiked with high concentrations of Human FE and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
