The QuickStep Human IL-8 (Interleukin-8) ELISA Kit is a powerful tool for measuring IL-8 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, enabling researchers to obtain accurate and reproducible results for a variety of research applications.Interleukin-8 is a key chemokine involved in inflammation and immune responses, playing a critical role in diseases such as cancer, inflammatory disorders, and infections. By accurately measuring IL-8 levels, researchers can gain valuable insights into the pathogenesis of these conditions and explore potential therapeutic targets. Overall, the QuickStep Human IL-8 (Interleukin-8) ELISA Kit is a valuable tool for researchers studying inflammation, immune responses, and various disease states where IL-8 is implicated. Its high sensitivity and specificity make it a reliable choice for quantifying IL-8 levels in human samples and advancing our understanding of complex biological processes.
Product Name:
Human IL-8 Quickstep ELISA Kit
SKU:
QSES028
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
