The QuickStep Monkey Cortisol ELISA Kit is specifically designed for the quantitative detection of cortisol levels in monkey serum, plasma, and saliva samples. This kit offers a high sensitivity and specificity, providing accurate and reliable results for research applications in the field of stress physiology and endocrinology.Cortisol is a key steroid hormone that plays a vital role in the body's response to stress, regulating metabolism, immune function, and stress responses. Abnormal cortisol levels have been associated with various health conditions, making it an important biomarker for studying stress-related disorders such as anxiety, depression, and adrenal disorders.
By using the QuickStep Monkey Cortisol ELISA Kit, researchers can easily measure cortisol levels in monkey samples, allowing for detailed analysis of the physiological effects of stress and the potential development of new therapeutic interventions. This kit is an essential tool for any laboratory conducting research on stress-related mechanisms in primates.
Product Name:
QuickStep Monkey Cortisol ELISA Kit
Product Code:
QSES040
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Monkey
Sensitivity:
1.13 ng/mL
Range:
3.13-200 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Cortisol
Application:
This ELISA kit applies to the in vitro quantitative determination of Cortisol concentrations in serum and plasma.
Specificity:
This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Monkey serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
9.61
16.80
94.49
9.56
15.96
103.65
Standard deviation
0.62
0.99
4.04
0.54
0.67
4.75
CV (%)
6.43
5.92
4.28
5.63
4.20
4.58
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
90-104
97
EDTA plasma(n=8)
90-103
96
Samples were spiked with high concentrations of Monkey Cortisol and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
