The QuickStep Mouse IgG2c (Immunoglobulin G2c) ELISA Kit is a cutting-edge assay crafted for the precise and quantitative detection of IgG2c levels in mouse biological samples. IgG2c serves as a vital immunoglobulin subtype involved in immune responses and immunomodulation processes in murine models. This ELISA kit features state-of-the-art technology and advanced sensitivity to accurately measure IgG2c concentrations, providing valuable insights into immune system dynamics and adaptive immune responses in mice. Researchers and scientists can rely on the QuickStep Mouse IgG2c ELISA Kit to deliver accurate and reproducible data, enabling comprehensive analyses of immunoglobulin profiles and immune function in mouse models. The kit's exceptional sensitivity and specificity ensure dependable results, making it an invaluable tool for immunology research, autoimmunity studies, and antibody-mediated responses. Manufactured under rigorous quality control standards, the QuickStep Mouse IgG2c ELISA Kit guarantees robust performance and user-friendly operation, making it an optimal choice for researchers seeking precise and reliable quantification of IgG2c levels. Explore the intricate world of immune responses and antibody dynamics in mouse models with this advanced ELISA kit.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
