The Mouse IgG2c (Immunoglobulin G2c) ELISA Kit is a powerful tool for accurately measuring IgG2c levels in mouse serum, plasma, and tissue homogenates. With its high sensitivity and specificity, this kit delivers dependable and consistent results, making it an excellent choice for various research applications.Immunoglobulin G2c is a subclass of the IgG antibody that plays a critical role in immune responses, particularly in antibody-dependent cellular cytotoxicity and immune complex formation. By measuring IgG2c levels, researchers can gain valuable insights into the immune system's functioning and its response to various pathogens and antigens.This ELISA kit is essential for studying autoimmune diseases, infectious diseases, and vaccine development, as it provides precise measurements of IgG2c levels, allowing researchers to better understand the immune response in mouse models. Trust the Mouse IgG2c ELISA Kit for accurate and reliable results in your research.
Product Name:
Mouse IgG2c (Immunoglobulin G2c) ELISA Kit
SKU:
MOES01681
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
