The Rat ADP (Adiponectin) ELISA Kit is specifically designed for the quantitative measurement of adiponectin levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and precision, guaranteeing accurate and consistent results for a variety of research purposes.Adiponectin is a key adipokine that plays a vital role in regulating glucose and lipid metabolism, as well as inflammation and insulin sensitivity. Dysregulation of adiponectin levels has been linked to various metabolic disorders such as obesity, type 2 diabetes, and cardiovascular diseases, making it a valuable marker for studying these conditions and potential therapeutic interventions. With easy-to-follow protocols and efficient assay performance, the Rat ADP ELISA Kit is a valuable tool for researchers exploring the role of adiponectin in metabolic diseases and seeking new insights into potential treatment strategies.
Product Name:
Rat ADP/Acrp30 (Adiponectin) Quickstep ELISA Kit
SKU:
QSES053
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
1 h 30 min
Sensitivity:
6.43 ng/mL
Detection range:
15.63-1000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
