The Rabbit anti His-Tag pAb (CABE068) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting His-tagged proteins in Western blot assays. The His tag, commonly added to recombinant proteins for purification and detection purposes, can be easily recognized by this antibody, allowing for efficient and accurate analysis.With its strong reactivity towards His-tagged proteins, this antibody is ideal for use in protein expression studies, protein purification processes, and protein-protein interaction studies.
This antibody is validated for use in WB, IF/ICC, IP applications and has demonstrated reactivity against Species independent samples.
Product Name:
Rabbit anti His-Tag pAb
SKU:
CABE068
Size:
20μL, 50μL, 100μL
Reactivity:
Species independent
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
HHHH HH
Tested Applications:
WBIF/ICCIP
Recommended Dilution:
WB
1:1000 - 1:5000
IF/ICC
1:50 - 1:200
IP
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
His, His tag, His-tag
Positive Sample:
293T
Observed MW:
35kDa
Consecutive histidine residues (usually 6 to 10 in length) are often inserted into the amino acid sequences of recombinant proteins. The resulting His-tagged proteins can be detected by using His antibodies.
Purification Method
Affinity purification
RRID
AB_2863794
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of extracts of normal 293T cells, 293T transfected with EPCAM Protein and 293T transfected with SOST Protein, using Rabbit anti His-Tag pAb (CABE068) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Immunofluorescence analysis of HeLa cells using Rabbit anti His-Tag pAb (CABE068) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of extracts of cells using His-Tag pAb (CABE068). Western blot was performed from the immunoprecipitate using His-Tag pAb (CABE068) at a dilition of 1:3000.
