**** The Rabbit MMP-13 (Matrix Metalloproteinase 13) ELISA Kit is a specialized assay meticulously crafted for the quantitative determination of Matrix Metalloproteinase 13 (MMP-13) levels in a variety of biological samples obtained from rabbit specimens.
MMP-13, also known as collagenase-3, plays a crucial role in extracellular matrix remodeling and degradation processes, particularly in the context of tissue repair, inflammation, and angiogenesis in rabbit models. Accurate measurement of MMP-13 expression levels is fundamental for unraveling its functions in physiological and pathological settings, including tissue remodeling, cartilage degradation, and matrix turnover in rabbits. This ELISA kit offers outstanding sensitivity and specificity, ensuring precise and reproducible outcomes for assessing MMP-13 concentrations. Crafted under rigorous quality standards, this kit delivers reliable performance and user-friendly protocols, making it an ideal tool for scientific investigations focusing on matrix metalloproteinases and their impact on tissue homeostasis in rabbits.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit MMP-13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit MMP-13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit MMP-13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit MMP-13. You can calculate the concentration of Rabbit MMP-13 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-101
83-98
97-108
Average (%)
96
90
102
1:4
Range (%)
91-105
82-94
95-113
Average (%)
97
88
103
1:8
Range (%)
90-103
86-98
98-110
Average (%)
96
93
105
1:16
Range (%)
87-102
83-94
99-111
Average (%)
94
88
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
100
EDTA plasma (n=5)
89-103
96
Cell culture media (n=5)
84-96
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
9.76
27.52
93.86
9.89
25.47
99.98
Standard deviation
0.54
1.31
5.14
0.56
1.26
4.68
C V (%)
5.53
4.76
5.48
5.66
4.95
4.68
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit MMP-13 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit MMP-13 in samples. No significant cross-reactivity or interference between Rabbit MMP-13 and analogues was observed.
