The Rabbit MMP-3 (Matrix Metalloproteinase 3) ELISA Kit is a specialized assay designed for the precise and quantitative detection of MMP-3 levels in various rabbit biological samples. MMP-3, also known as stromelysin-1, is an important protease involved in the degradation of extracellular matrix components, specifically collagen. It plays a crucial role in tissue remodeling processes and is implicated in various physiological and pathological conditions such as wound healing, development, and inflammatory responses.
This ELISA kit offers outstanding sensitivity and specificity, ensuring accurate and reproducible results in the assessment of MMP-3 concentrations. Manufactured under strict quality control standards, this kit guarantees robust performance and ease of use, making it an ideal choice for researchers investigating the role of MMP-3 in tissue homeostasis, remodeling, and pathological conditions.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit MMP-3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit MMP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit MMP-3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit MMP-3. You can calculate the concentration of Rabbit MMP-3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-99
90-102
99-115
Average (%)
94
97
105
1:4
Range (%)
88-99
82-96
88-102
Average (%)
93
88
94
1:8
Range (%)
89-103
86-98
82-95
Average (%)
94
91
87
1:16
Range (%)
88-103
81-92
84-100
Average (%)
96
87
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-109
99
EDTA plasma (n=5)
92-103
98
Cell culture media (n=5)
89-103
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.51
1.29
4.29
0.56
1.18
4.09
Standard deviation
0.03
0.05
0.19
0.03
0.06
0.15
C V (%)
5.88
3.88
4.43
5.36
5.08
3.67
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit MMP-3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit MMP-3 in samples. No significant cross-reactivity or interference between Rabbit MMP-3 and analogues was observed.
