The Rabbit TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit is a cutting-edge assay designed for the precise quantitative analysis of TNF-α levels in a range of rabbit biological samples. TNF-α, a pivotal pro-inflammatory cytokine, plays a fundamental role in a variety of cellular functions, from cell proliferation to immune regulation.
Its involvement in inflammatory processes, immune responses, and disease pathology has made it a key target for research and therapeutic development. This ELISA kit empowers researchers to accurately determine TNF-α concentrations, providing valuable insights into the role of this cytokine in inflammatory conditions, autoimmune disorders, and infections. By quantifying TNF-α levels, researchers can deepen their understanding of its function in disease mechanisms, supporting the development of targeted treatment strategies and potential therapeutic interventions. Manufactured to the highest standards, the Rabbit TNF-α ELISA Kit from Assay Genie ensures exceptional sensitivity, specificity, and reproducibility, delivering dependable results for research applications. With easy-to-use protocols and robust performance, this kit is an excellent choice for scientists investigating TNF-α biology and its implications in rabbit model studies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit TNF-α. You can calculate the concentration of Rabbit TNF-α in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-108
88-102
94-109
Average (%)
102
94
101
1:4
Range (%)
92-106
85-99
86-99
Average (%)
98
91
93
1:8
Range (%)
89-103
85-98
87-99
Average (%)
94
91
94
1:16
Range (%)
90-105
80-92
86-96
Average (%)
97
86
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-109
101
EDTA plasma (n=5)
92-109
99
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
49.73
154.44
395.12
47.58
159.52
432.48
Standard deviation
2.85
6.49
13.0
2.45
8.87
22.79
C V (%)
5.73
4.2
3.29
5.15
5.56
5.27
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Rabbit TNF-α concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rabbit TNF-α in samples. No significant cross-reactivity or interference between Rabbit TNF-α and analogues was observed.
